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</div><div>　　實驗原理：</div><div>　　人ELISA檢測試劑盒應用雙抗體夾心法測定標本中待測物質水平。用純化的待測物質抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入待測物質，再與HRP標記的待測物質抗體結合，形成抗體-抗原-酶標抗體復合物，經過洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的待測物質呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中待測物質濃度。</div><div> </div><div>　　人ELISA檢測試劑盒的計算：</div><div>　　以標準物的濃度為橫坐標，OD值為縱坐標，在坐標紙上繪出標準曲線，根據樣品的OD值由標準曲線查出相應的濃度；再乘以稀釋倍數；或用標準物的濃度與OD值計算出標準曲線的直線回歸方程式，將樣品的OD值代入方程式，計算出樣品濃度，再乘以稀釋倍數，即為樣品的實際濃度。</div><div> </div><div>　　兔ELISA試劑盒液體類標本：包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清等。</div><div>　　1、血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右。仔細收集上清。保存過程中如有沉淀形成，應再次離心。</div><div>　　2、血漿：應根據標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右。仔細收集上清。保存過程中如有沉淀形成，應再次離心。</div><div>　　3、尿液：用無菌管收集。離心20分鐘左右。仔細收集上清。保存過程中如有沉淀形成，應再次離心。胸腹水、腦脊液參照此實行。</div><div>　　4、細胞培養上清：檢測分泌性的成份時，用無菌管收集。離心20分鐘左右。仔細收集上清。檢測細胞內的成份時，用PBS（PH7.2-7.4）稀釋細胞懸液，細胞濃度達到100萬/ml左右。通過反復凍融，以使細胞破壞并放出細胞內成份。離心20分鐘左右。仔細收集上清。保存過程中如有沉淀形成，應再次離心。</div><div>　　5、組織標本：切割標本后，稱取重量。加入一定量的PBS，PH7.4。用液氮迅速冷凍保存備用。標本融化后仍然保持2-8℃的溫度。加入一定量的PBS（PH7.4），用手工或勻漿器將標本勻漿充分。離心20分鐘左右。仔細收集上清。分裝后一份待檢測，其余冷凍備用。</div> </div> </div> <div class="artview_prev_next"> <p class="artview_prev"><span></span>上一篇：<a href="/News-1449741.html">小鼠表皮生長因子(EGF)elisa試劑盒是如何進行樣本處理的？</a></p> <p class="artview_next"><span></span>下一篇：<a href="/News-1440897.html">上?；钌镫p十一elisa試劑盒優惠活動</a></p> </div> </div> </div> </div> <div id="clear"></div> <footer>  <div class="foot1"> <div class="foot1_in clear clearfix"> <ul class="foot_nav"> <li> 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