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target="_blank">人降鈣素(CT)elisa試劑盒</a></strong>測定現通常為采用手工操作的以微孔板條為固相的測定模式，測定操作非常簡單，一般涉及到標本的收集保存、試劑準備、加樣、溫育、洗板、顯色、比色、結果判斷和結果報告及解釋等方面，其中任一步驟的不當都會影響測定結果，且尤以加樣、溫育和洗板等步驟為甚。</div><div> </div><div>　　檢測原理：</div><div>　　人降鈣素(CT)elisa試劑盒采用雙抗體一步夾心法酶聯免疫吸附試驗(ELISA)。往預先包被抗體的包被微孔中，依次加入標本、標準品、HRP標記的檢測抗體，經過溫育并洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中呈正相關。用酶標儀在450nm波長下測定吸光度(OD值)，計算樣品濃度。</div><div> </div><div>　　人降鈣素(CT)elisa試劑盒樣品收集、處理及保存方法：</div><div>　　1、血清：使用不含熱原和內毒素的試管，操作過程中避免任何細胞刺激，收集血液后，3000轉離心10分鐘將血清和紅細胞迅速小心地分離；</div><div>　　2、血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉離心30分鐘取上清；</div><div>　　3、細胞上清液：3000轉離心10分鐘去除顆粒和聚合物；</div><div>　　4、組織勻漿：將組織加入適量生理鹽水搗碎。3000轉離心10分鐘取上清；</div><div>　　5、保存：如果樣本收集后不及時檢測，請按一次用量分裝，凍存于-20℃，避免反復凍融，在室溫下解凍并確保樣品均勻地充分解。</div> </div> </div> <div class="artview_prev_next"> <p class="artview_prev"><span></span>上一篇：<a href="/Article-3274952.html">實驗室用人elisa試劑盒在日常使用中有7大特點</a></p> <p class="artview_next"><span></span>下一篇：<a href="/Article-3231957.html">解析！血清elisa試劑盒的操作流程</a></p> </div> </div> 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