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class="eja">色素類</a></li> <li class="ej"><a href="/SonList-2371522.html" class="eja">氨基酸類</a></li> <li class="ej"><a href="/SonList-2371520.html" class="eja">碳水化合物</a></li> <li class="ej"><a href="/SonList-2371519.html" class="eja">維生素類</a></li> </ul> </div> </li> <li class="yj"><a href="/ParentList-2383399.html" class="yja" onmouseover="showsubmenu('PCR試劑盒')">PCR試劑盒</a> <div id="submenu_PCR試劑盒" style="display:none"> <ul> <li class="ej"><a href="/SonList-2383400.html" class="eja">PCR檢測試劑盒</a></li> </ul> </div> </li> </ul> </div> </div> <div class="ny_right"> <div class="news_detail"> <h1 class="detail_title">解析！血清elisa試劑盒的操作流程</h1> <div class="details_tongji">發布時間：2022-12-23　點擊量：<span id="Click">187</span> </div> <div class="n_con"> <div>　　<strong><a href="http://www.xuzhoushixinda.com/Products-37043602.html" target="_blank">血清elisa試劑盒</a></strong>是基于經典的雙抗夾心ELISA方法，利用HRP偶聯的抗體，催化TMB產生顏色物質，基于標準曲線，計算出樣品中人抵抗素的含量。</div><div> </div><div>　　試劑盒特點有：</div><div>　　1、高效、靈敏、特異的抗體；</div><div>　　2、穩定的重復性和可靠性；</div><div>　　3、吸附性能好，空白值低，孔底透明度高的固相載體；</div><div>　　4、適用血清、血漿、組織勻漿液、細胞培養上清液、尿液等等多種標本類型；</div><div>　　5、節省實驗經費。</div><div> </div><div>　　血清elisa試劑盒的操作流程如下：</div><div>　　1、血清總補體ELISA試劑盒待測樣品孔中每孔加入待測樣品100μl，每種樣品設3個平行孔；設兩個陰性對照孔，每孔加未處理組的細胞裂解液100μl；另設一個空白對照孔，加入純細胞裂解液100μl。</div><div>　　2、酶標板置4℃，包被過夜。</div><div>　　3、洗板：吸干孔內反應液，產品僅用于科研用洗滌液過洗一遍，之后將洗滌液注滿板孔，浸泡1-2分鐘，間歇搖動。甩去孔內液體后在吸水紙上拍干。重復洗滌3-4次。</div><div>　　4、陰性對照孔每孔加入PBS 50μl，樣品孔及空白孔每孔加入1:500稀釋的兔抗人AIF抗體工作液50μl。</div><div>　　5、酶標板置37℃培養箱的濕盒內，孵育60min。</div><div>　　6、洗板，同(4)。</div><div>　　7、每孔加1:5000稀釋的HRP-標記的山羊抗兔抗體工作液 100μl。</div><div>　　8、酶標板置37℃培養箱的濕盒內，孵育60min。</div><div>　　9、洗板，同(4)。</div><div>　　10、每孔加TMB顯色液 100μl，輕輕混勻10s，置37℃暗處反應15-20min。</div><div>　　11、每孔加100μl 2mol/L H2SO4終止反應。</div><div>　　12、分別測450nm吸光值W1和630nm吸光值W2，最終測得的OD值為兩者之差(W1-W2)，以減少由容器上的劃痕或指印等造成的光干擾。</div><div>　　13、數據處理：在得出標本(S)和陰性對照(N)的 OD值后，計算S/N值。S/N≥2.1為陽性判定標準。</div><div> </div> </div> </div> <div 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